fgf1 nm 010197 mouse Search Results


fgf1 nm 010197 mouse  (OriGene)
93
OriGene fgf1 nm 010197 mouse
Bmal1 regulates <t>FGF1,</t> which supports mitochondrial respiration in hepatocytes. A , mRNA ( right ) and protein abundance ( left ) of FGF1 protein in liver. Two-way ANOVA with Tukey’s multiple comparisons test, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001, n = 4. B , Western blot for FGF1 in liver at the indicated diurnal time points. Biological replicates are shown ( bottom ) and quantified ( top ). One-way ANOVA with Fisher’s LSD test, ∗ p < 0.05 and ∗∗ p < 0.01, n = 3. Liver-RE = Bmal1 stopFL/stopFL ; Alfp -Cre tg/0 , hepatocyte-specific reconstitution of Bmal1 . C and D , knockdown or overexpression of Fgf1 in AML12 hepatocytes. si-Ctrl = scrambled sequence; both si-Fgf1 #1 and #2 target Fgf1 but by different sequences. pCMV6-Empty = control vector without Fgf1 open reading frame clone. Left , qPCR, Student’s t test, ∗∗ p < 0.01 and ∗∗∗ p < 0.001, n = 3. Middle , oxygen consumption rate in AML12 hepatocytes. Arrow indicates application of 0.5 μM carbonyl cyanide- p -trifluoromethoxyphenylhydrazone (FCCP) and 1 μM oligomycin. Right , quantification of middle . Basal and stressed values are averages of data points before and after drug application, respectively. Two-way ANOVA with Dunnett’s post hoc test, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001, n = 5 to 6. E , mRNA levels of FGF receptor (FGFR) isoforms in WT (Alfp-Cre+ and Hsa-Cre+) liver. F , measurement of oxygen consumption rate as in D and E . AML12 cells were treated for 24 h with vehicle control (Veh) or the indicated concentration of the pan-FGFR inhibitor AZD4547. Two-way ANOVA with Tukey’s post hoc test, ∗ p < 0.05, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001, n = 5 to 6. All mRNA plots are normalized to WT ZT 0, n = 3 per time point per genotype. AL, ad libitum feeding; AML12, alpha mouse liver 12; FGF, fibroblast growth factor; LSD, least significant difference; ns, not significant; qPCR, quantitative PCR; TRF, time-restricted feeding; ZT, zeitgeber time.
Fgf1 Nm 010197 Mouse, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fgf1 nm 010197 mouse/product/OriGene
Average 93 stars, based on 1 article reviews
fgf1 nm 010197 mouse - by Bioz Stars, 2026-03
93/100 stars
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mr201152  (OriGene)
93
OriGene mr201152
Bmal1 regulates <t>FGF1,</t> which supports mitochondrial respiration in hepatocytes. A , mRNA ( right ) and protein abundance ( left ) of FGF1 protein in liver. Two-way ANOVA with Tukey’s multiple comparisons test, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001, n = 4. B , Western blot for FGF1 in liver at the indicated diurnal time points. Biological replicates are shown ( bottom ) and quantified ( top ). One-way ANOVA with Fisher’s LSD test, ∗ p < 0.05 and ∗∗ p < 0.01, n = 3. Liver-RE = Bmal1 stopFL/stopFL ; Alfp -Cre tg/0 , hepatocyte-specific reconstitution of Bmal1 . C and D , knockdown or overexpression of Fgf1 in AML12 hepatocytes. si-Ctrl = scrambled sequence; both si-Fgf1 #1 and #2 target Fgf1 but by different sequences. pCMV6-Empty = control vector without Fgf1 open reading frame clone. Left , qPCR, Student’s t test, ∗∗ p < 0.01 and ∗∗∗ p < 0.001, n = 3. Middle , oxygen consumption rate in AML12 hepatocytes. Arrow indicates application of 0.5 μM carbonyl cyanide- p -trifluoromethoxyphenylhydrazone (FCCP) and 1 μM oligomycin. Right , quantification of middle . Basal and stressed values are averages of data points before and after drug application, respectively. Two-way ANOVA with Dunnett’s post hoc test, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001, n = 5 to 6. E , mRNA levels of FGF receptor (FGFR) isoforms in WT (Alfp-Cre+ and Hsa-Cre+) liver. F , measurement of oxygen consumption rate as in D and E . AML12 cells were treated for 24 h with vehicle control (Veh) or the indicated concentration of the pan-FGFR inhibitor AZD4547. Two-way ANOVA with Tukey’s post hoc test, ∗ p < 0.05, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001, n = 5 to 6. All mRNA plots are normalized to WT ZT 0, n = 3 per time point per genotype. AL, ad libitum feeding; AML12, alpha mouse liver 12; FGF, fibroblast growth factor; LSD, least significant difference; ns, not significant; qPCR, quantitative PCR; TRF, time-restricted feeding; ZT, zeitgeber time.
Mr201152, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mr201152/product/OriGene
Average 93 stars, based on 1 article reviews
mr201152 - by Bioz Stars, 2026-03
93/100 stars
  Buy from Supplier

Image Search Results


Bmal1 regulates FGF1, which supports mitochondrial respiration in hepatocytes. A , mRNA ( right ) and protein abundance ( left ) of FGF1 protein in liver. Two-way ANOVA with Tukey’s multiple comparisons test, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001, n = 4. B , Western blot for FGF1 in liver at the indicated diurnal time points. Biological replicates are shown ( bottom ) and quantified ( top ). One-way ANOVA with Fisher’s LSD test, ∗ p < 0.05 and ∗∗ p < 0.01, n = 3. Liver-RE = Bmal1 stopFL/stopFL ; Alfp -Cre tg/0 , hepatocyte-specific reconstitution of Bmal1 . C and D , knockdown or overexpression of Fgf1 in AML12 hepatocytes. si-Ctrl = scrambled sequence; both si-Fgf1 #1 and #2 target Fgf1 but by different sequences. pCMV6-Empty = control vector without Fgf1 open reading frame clone. Left , qPCR, Student’s t test, ∗∗ p < 0.01 and ∗∗∗ p < 0.001, n = 3. Middle , oxygen consumption rate in AML12 hepatocytes. Arrow indicates application of 0.5 μM carbonyl cyanide- p -trifluoromethoxyphenylhydrazone (FCCP) and 1 μM oligomycin. Right , quantification of middle . Basal and stressed values are averages of data points before and after drug application, respectively. Two-way ANOVA with Dunnett’s post hoc test, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001, n = 5 to 6. E , mRNA levels of FGF receptor (FGFR) isoforms in WT (Alfp-Cre+ and Hsa-Cre+) liver. F , measurement of oxygen consumption rate as in D and E . AML12 cells were treated for 24 h with vehicle control (Veh) or the indicated concentration of the pan-FGFR inhibitor AZD4547. Two-way ANOVA with Tukey’s post hoc test, ∗ p < 0.05, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001, n = 5 to 6. All mRNA plots are normalized to WT ZT 0, n = 3 per time point per genotype. AL, ad libitum feeding; AML12, alpha mouse liver 12; FGF, fibroblast growth factor; LSD, least significant difference; ns, not significant; qPCR, quantitative PCR; TRF, time-restricted feeding; ZT, zeitgeber time.

Journal: Molecular & Cellular Proteomics : MCP

Article Title: Impact of Bmal1 Rescue and Time-Restricted Feeding on Liver and Muscle Proteomes During the Active Phase in Mice

doi: 10.1016/j.mcpro.2023.100655

Figure Lengend Snippet: Bmal1 regulates FGF1, which supports mitochondrial respiration in hepatocytes. A , mRNA ( right ) and protein abundance ( left ) of FGF1 protein in liver. Two-way ANOVA with Tukey’s multiple comparisons test, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001, n = 4. B , Western blot for FGF1 in liver at the indicated diurnal time points. Biological replicates are shown ( bottom ) and quantified ( top ). One-way ANOVA with Fisher’s LSD test, ∗ p < 0.05 and ∗∗ p < 0.01, n = 3. Liver-RE = Bmal1 stopFL/stopFL ; Alfp -Cre tg/0 , hepatocyte-specific reconstitution of Bmal1 . C and D , knockdown or overexpression of Fgf1 in AML12 hepatocytes. si-Ctrl = scrambled sequence; both si-Fgf1 #1 and #2 target Fgf1 but by different sequences. pCMV6-Empty = control vector without Fgf1 open reading frame clone. Left , qPCR, Student’s t test, ∗∗ p < 0.01 and ∗∗∗ p < 0.001, n = 3. Middle , oxygen consumption rate in AML12 hepatocytes. Arrow indicates application of 0.5 μM carbonyl cyanide- p -trifluoromethoxyphenylhydrazone (FCCP) and 1 μM oligomycin. Right , quantification of middle . Basal and stressed values are averages of data points before and after drug application, respectively. Two-way ANOVA with Dunnett’s post hoc test, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001, n = 5 to 6. E , mRNA levels of FGF receptor (FGFR) isoforms in WT (Alfp-Cre+ and Hsa-Cre+) liver. F , measurement of oxygen consumption rate as in D and E . AML12 cells were treated for 24 h with vehicle control (Veh) or the indicated concentration of the pan-FGFR inhibitor AZD4547. Two-way ANOVA with Tukey’s post hoc test, ∗ p < 0.05, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001, n = 5 to 6. All mRNA plots are normalized to WT ZT 0, n = 3 per time point per genotype. AL, ad libitum feeding; AML12, alpha mouse liver 12; FGF, fibroblast growth factor; LSD, least significant difference; ns, not significant; qPCR, quantitative PCR; TRF, time-restricted feeding; ZT, zeitgeber time.

Article Snippet: For overexpression experiments, cells were transfected with 100 ng Fgf1 (NM_010197) mouse-tagged ORF Clone (ORIGENE; catalog no.: MR201152) or control empty vector using Lipofectamine 3000 Transfection Reagent (Invitrogen; catalog no.: L3000001) according to the manufacturer’s instructions.

Techniques: Western Blot, Knockdown, Over Expression, Sequencing, Control, Plasmid Preparation, Concentration Assay, Real-time Polymerase Chain Reaction